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For convenient immunofluorescence-based analysis, we populated the four available imaging channels by staining for parasite vacuoles (using the Fluorescein-conjugated lectin Vicia villosa lectin or VVL), female parasites (detected with an antibody against female-specific oocyst wall protein COWP1), host-derived actin ‘pedestals’ formed beneath parasite vacuoles (detected by fluorescently-conjugated phalloidin), and host cell nuclei (using Hoechst) (Fig S1E)
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