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Dose selection by research model Representative doses by endpoint: Cardiac post-MI (mouse): 150 µg/kg IP, often single or repeated dosing in acute protocols Cardiac post-MI (rat): higher absolute doses, scaled by species weight Skeletal muscle injury (rat): 2-10 mg per animal, IM or SC, weekly for 2-6 weeks Dermal wound healing: topical or systemic dosing depending on design Corneal wound healing: topical administration in eye drops (specialised formulation) CNS / stroke models: typically IP or SC systemic administration 9

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Role of intestinal microbiota and metabolites on gut homeostasis and human diseases