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The solvent was dried by heating, and the formed lipid film was hydrated with 0.5 mL of PBS solutions of Mb at 40 °C for 30 min and then sonicated in a water bath with 100% output power for 30 min, followed by filtration through a 0.22 μm syringe filter (Millipore, USA) to remove the large MLVs

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After applying, wash your hands thoroughly with soap (you don’t want any residual cream on your fingers to accidentally get in your eyes or touch other areas)