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Subsequently, cDNA synthesis from the isolated RNA was carried out using cDNA EcoDry Premix (Takara, #639549, San Jose, CA, USA) in accordance with the manufacturer’s guidelines

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Although HDACis are capable of restoring Class I-III G6PD variant activity to that of WT or greater in erythroid precursor cells, histone deacetylation is important for DNA compression, nuclear extrusion, and erythroid maturation [83-85]