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The absolute amounts of metabolites in clinical colorectal tumor samples (n=275), their matched normal tissues (n=275) (23) and cell lines (RCC4 VHL−/− and RCC4 VHL+/+) were measured using capillary electrophoresis-triple quadrupole/time-of-flight MS at Keio University (Tsuruoka, Japan), according to the methods of Yuan et al (24) and Soga et al (25–27)

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Quantitative real-time PCR for mRNA levels Quantitative real-time PCR (qPCR) was run using standard methods with either Taqman probes or specific primers (Supplementary Table 2)