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Briefly, hematopoietic progenitor cells were generated from iPSCs using STEMdiff Hematopoietic kit (STEMCELL Technologies) and cultured in microglia growth medium supplemented with 100 ng/mL interleukin-34, 50 ng/mL tumor growth factor-beta and 25 ng/mL macrophage colony-stimulating factor (Peprotech) for 25 days, following which 100 ng/mL cluster of differentiation 200 (Abcam) and C-X3-C motif chemokine ligand 1 (Peprotech) were also added to the culture
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