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After PBS washing, HRP Polymer Quanto (TL-125-QPH, Thermo Fisher Scientific, USA) was applied, and immunoreactivity was visualized with DAB chromogen (TA-125-HA, Thermo Fisher Scientific, USA), followed by hematoxylin counterstaining, in accordance with previously described standard immunohistochemical procedures
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Accordingly, in muscle-to-bone reattachment, there were mature fibers, oriented parallel with the bone axis and in close contact with bone
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